Can I pause a play through?

Yes - you can pause a play through using the Pause button in the upper left corner of the screen.

This will suspend any video and play through until you press the resume button. Any timing of your play through will also be paused while you are on the Paused screen.

Note: The Pause button is not available in Research mode as the timing of play throughs is an integral part of the research data and so no interruptions are allowed.